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Regulation of Lipid Homeostasis

  • Burr R, Stewart EV, Espenshade PJ. 2017. Coordinate Regulation of Yeast Sterol Regulatory Element-Binding Protein (SREBP) and Mga2 Transcription Factors. J Biol Chem. [ePub ahead of print] [LINK To PUBMED]
  • Gong X, Qian H, Shao W, Li J, Wu J, Liu JJ, Li W, Wang HW, Espenshade P, Yan N. 2016. Complex structure of the fission yeast SREBP-SCAP binding domains reveals an oligomeric organization. Cell Res. 26(11):1197-1211 [LINK To PUBMED]
  • Hwang J, Ribbens D, Raychaudhuri S, Cairns L, Gu H, Frost A, Urban S, Espenshade PJ. 2016. A Golgi rhomboid protease Rbd2 recruits Cdc48 to cleave yeast SREBP. EMBO 35(21):2332-2349 [LINK To PUBMED]
  • Shao W, Machamer CE, and Espenshade PJ. 2016. Fatostatin blocks ER exit of SCAP but inhibits cell growth in a SCAP-independent manner. J Lipid Res. 57(8): 1564-73 [LINK To PUBMED]
  • Burr R, Stewart EV, Shao W, Zhao S, Hannibal-Bach HK, Ejsing CS, and Espenshade PJ. 2016. Mga2 transcription factor regulates an oxygen-responsive lipid homeostasis pathway in fission yeast. J Biol Chem. 291(23):12171-83 [LINK To PUBMED]
  • Raychaudhuri S, and Espenshade PJ. 2015. Endoplasmic Reticulum Exit of Golgi-resident Defective for SREBP Cleavage (Dsc) E3 Ligase Complex Requires Its Activity. J Biol Chem. 290:23 14430-40 [LINK To PUBMED]
  • Gong X, Li J, Shao W, Wu J, Qian H, Ren R, Espenshade P, and Yan N. 2015. Structure of the WD40 domain of SCAP from fission yeast reveals the molecular basis for SREBP recognition. Cell Res. 25:4 401-411 [LINK To PUBMED]
  • Tong Z, Kim MS, Pandey A, and Espenshade PJ. 2014. Identification of Candidate Substrates for the Golgi Tul1 E3 Ligase Using Quantitative diGly Proteomics in Yeast. Mol Cell Proteomics 13:2871-82 [LINK To PUBMED]
  • Shao, W and Espenshade, PJ. 2014. Sterol Regulatory Element-binding Protein (SREBP) Cleavage Regulates Golgi-to-ER Recycling of SREBP Cleavage Activating Protein (SCAP). J Biol Chem. 289:7547-57 [LINK To PUBMED]
  • Brookheart RT, Lee CY, Espenshade PJ. 2013. Casein kinase 1 regulates sterol regulatory element-binding protein (SREBP) to control sterol homeostasis. J Biol Chem. 289:2725-35 [LINK To PUBMED]
  • Lloyd SJ, Raychaudhuri S, Espenshade PJ. 2013. Subunit architecture of the Golgi Dsc E3 ligase required for sterol regulatory element-binding protein (SREBP) cleavage in fission yeast. J Biol Chem. 288:21043-54. [LINK To PUBMED]
  • Cheung R, Espenshade PJ. 2013. Structural requirements for sterol regulatory element-binding protein (SREBP) cleavage in fission yeast. J Biol Chem. 288:20351-60. [LINK To PUBMED]
  • Stewart EV, Lloyd SJ, Burg JS, Nwosu CC, Lintner RE, Daza R, Russ C, Ponchner K, Nusbaum C, Espenshade PJ. 2012. Yeast sterol regulatory element-binding protein (SREBP) cleavage requires Cdc48 and Dsc5, a ubiquitin regulatory X domain-containing subunit of the Golgi Dsc E3 ligase. J Biol Chem. 287:672-81. [LINK To PUBMED]
  • Ryan CJ, Roguev A, Patrick K, Xu J, Jahari H, Tong Z, Beltrao P, Shales M, Qu H, Collins SR, Kliegman JI, Jiang L, Kuo D, Tosti E, Kim HS, Edelmann W, Keogh MC, Greene D, Tang C, Cunningham P, Shokat KM, Cagney G, Svensson JP, Guthrie C, Espenshade PJ, Ideker T, Krogan NJ. 2012. Hierarchical modularity and the evolution of genetic interactomes across species. Mol Cell. 46:691-704. [LINK To PUBMED]
  • Burg JS, Espenshade PJ. 2011. Glucose controls phosphoregulation of HMG-COA reductase through the PP2A-related phosphatase Ppe1 and Insig in fission yeast. J. Biol. Chem. 286:27139-46. [LINK To PUBMED]
  • Porter JR, Burg JS, Espenshade PJ, Iglesias PA. 2012. Identifying a static nonlinear structure in a biological system using noisy, sparse data. J Theor Biol. 300:232-41. [LINK To PUBMED]
  • Stewart EV, Nwosu CC, Tong Z, Roguev A, Cummins TD, Kim DU, Hayles J, Park HO, Hoe KL, Powell DW, Krogan NJ, Espenshade PJ. 2011. Yeast SREBP cleavage activation requires the Golgi Dsc E3 ligase complex. Mol. Cell 42:160-171. [LINK To PUBMED]
  • Porter JR, Burg JS, Espenshade PJ*, Iglesias PA*. 2010. Ergosterol regulates SREBP cleavage in fission yeast. J. Biol. Chem. 285:41051-61. *Corresponding author. [LINK To PUBMED]
  • Hughes BT, Nwosu CC, Espenshade PJ. 2009. Degradation of sterol regulatory element-binding protein precursor requires the endoplasmic reticulum-associated degradation components Ubc7 and Hrd1 in fission yeast. J Biol Chem. 284:20512-21 [LINK To PUBMED]
  • Burg JS, Powell DW, Chai R, Hughes AL, Link AJ, Espenshade PJ. 2008. Insig regulates HMG-CoA reductase by controlling enzyme phosphorylation in fission yeast. Cell Metabolism 8:522-31. [LINK To PUBMED]
  • Hughes, A.L., Stewart, E.V. and Espenshade, P.J. 2008. Identification of 23 mutations in fission yeast Scap that constitutively activate SREBP. J. Lipid Research 49:2001-2012. [LINK To PUBMED]
  • Sehgal, A, Hughes, B.T., Espenshade, P.J. 2008. Oxygen-dependent, alternative promoter controls translation of tco1+ in fission yeast. Nucleic Acids Research 36:2024-2031. [LINK To PUBMED]
  • Sehgal, A., C.Y. Lee, P.J. Espenshade. 2007. SREBP controls oxygen-dependent mobilization of retrotransposons in fission yeast. PLoS Genet. 3:1389-1396. [LINK To PUBMED]
  • Hughes, A.L., C.Y. Lee, C.M. Bien, P.J. Espenshade. 2007. 4-Methyl sterols regulate fission yeast SREBP-Scap under low oxygen and cell stress. J. Biol. Chem. 282:24388-96. [LINK To PUBMED]
  • Hughes, A.L., D.W. Powell, M. Bard, J. Eckstein, R. Barbuch, A.J. Link, and P.J. Espenshade. 2007. Dap1/PGRMC1 binds and regulates cytochrome P450 enzymes. Cell Metabolism 5:143-149. [LINK To PUBMED]
  • Todd, B.L., E.V. Stewart, J.S. Burg, A.L. Hughes, P.J. Espenshade. 2006. Sterol regulatory element binding protein is a principal regulator of anaerobic gene expression in fission yeast. Mol Cell. Biol. 26:2817-2831. [LINK To PUBMED]
  • Hughes, A.L., B.L. Todd, and P.J. Espenshade. 2005. SREBP pathway responds to sterols and functions as an oxygen sensor in fission yeast. Cell 120:831-842. [LINK To PUBMED]

Oxygen Sensing

  • Porter JR, Lee CY, Espenshade PJ, Iglesias PA. 2012. Regulation of SREBP during hypoxia requires Ofd1-mediated control of both DNA binding and degradation. Mol Biol Cell. 23:3764-74. [LINK To PUBMED]
  • Lee CY, Yeh TL, Hughes BT, Espenshade PJ. 2011. Regulation of the Sre1 hypoxic transcription factor by oxygen-dependent control of DNA binding. Mol Cell. 44:225-34. [LINK To PUBMED]
  • Yeh TL, Lee CSY, Amzel LM, Espenshade PJ*, and Bianchet MB*. 2011. The hypoxic regulator of sterol synthesis Nro1 is a nuclear import adaptor. Structure 19:503-14. *Corresponding author. [LINK To PUBMED]
  • Lee, C.Y., Stewart, E.V., Hughes, B.T. and Espenshade, P.J. 2009. Oxygen-dependent binding of Nro1 to the prolyl hydroxylase Ofd1 regulates SREBP degradation in yeast. EMBO J. 28:135-43. [LINK To PUBMED]
  • Hughes, B.T. and Espenshade, P.J. 2008. Oxygen-regulated degradation of fission yeast SREBP by Ofd1, a prolyl hydroxylase family member. EMBO J. 27:1491-1501. [LINK To PUBMED]

Pathogenic Fungi

  • Bien CM, Chang YC, Nes WD, Kwon-Chung KJ, and Espenshade PJ. 2009. Cryptococcus neoformans Site-2 protease is required for virulence and survival in the presence of azole drugs. Mol. Microbiol. 74:672-690. [LINK To PUBMED]
  • Chang YC, Ingavale SS, Bien C, Espenshade PJ, and Kwon-Chung KJ. 2009. Conservation of the SREBP pathway and its pathobiological importance in Cryptococcus neoformans. Eukaryot Cell 8:1770-1779. [LINK To PUBMED]
  • Lee, H., C.M. Bien, A.L. Hughes, P.J. Espenshade, K.J. Kwon-Chung. 2007. Cobalt chloride, a hypoxia-mimicking agent, targets sterol synthesis in the pathogenic fungus Cryptococcus neoformans. Mol. Microbiol. 65:1018-1033. [LINK To PUBMED]
  • Chang, Y.C., C.M. Bien, H. Lee, P.J. Espenshade, K.J. Kwon-Chung. 2007. Sre1p, a regulator of oxygen sensing and sterol homeostasis, is required for virulence in Cryptococcus neoformans. Mol. Microbiol. 64:614-29. [LINK To PUBMED]

Reviews, Methods, and Chapters

  • Hwang J and Espenshade PJ. 2016. Proximity-dependent biotin labeling in yeast using the engineered ascorbate peroxidase APEX2. Biochem J. 473(16):2463-9. [LINK To PUBMED]
  • Shao W, and Espenshade PJ. 2015. Sugar Makes Fat by Talking to SCAP. Cancer Cell 28:5 548-9 [LINK To PUBMED]
  • Shao W, Espenshade PJ. 2012. Expanding roles for SREBP in metabolism. Cell Metab. 16:414-9. [LINK To PUBMED]
  • Raychaudhuri S, Young BP, Espenshade PJ, Loewen C Jr. 2012. Regulation of lipid metabolism: a tale of two yeasts. Curr Opin Cell Biol. 24:502-8. [LINK To PUBMED]
  • Espenshade PJ. . Cholesterol Synthesis and Regulation. In: W.J. Lennarz and M.D. Lane (eds.) The Encyclopedia of Biological Chemistry, Elsevier Inc., Oxford.
  • Burg JS, Espenshade PJ. 2011. Regulation of HMG-CoA reductase in mammals and yeast. Prog. Lipid Res. 50:403–410. [LINK To PUBMED]
  • Bien CM and Espenshade PJ. 2010. Sterol regulatory element binding proteins in fungi: Hypoxic transcription factors linked to pathogenesis. Eukaryotic Cell. 9:352-9. [LINK To PUBMED]
  • Osborne TF, Espenshade PJ. 2009. Evolutionary conservation and adaptation in the mechanism that regulates SREBP action: what a long, strange tRIP it’s been. Genes Dev. 22:2578-81 [LINK To PUBMED]
  • Espenshade, P.J., A.L. Hughes. 2007. Regulation of sterol synthesis in eukaryotes. Annu. Rev. Genet. 41:401-427. [LINK To PUBMED]
  • Espenshade, P.J. 2006. SREBPs: sterol-regulated transcription factors. J. Cell Sci. 119:973-976. [LINK To PUBMED]
  • Espenshade, P.J., J.L. Goldstein, and M.S. Brown. 2003. SREBPs: Gene Regulation through Controlled Protein Trafficking. In Handbook of Cellular Signaling (R. Bradshaw and E. Dennis, eds.). Academic Press, San Diego.